Pyruvate Dehydrogenase (PDH) Activity Assay Kit

Catalog No. ABIN7850088

Quick Overview for Pyruvate Dehydrogenase (PDH) Activity Assay Kit (ABIN7850088)

Target: PDP (Pyruvate Dehydrogenase Phosphatase (PDP))

Detection Method: Colorimetric

Application: Biochemical Assay (BCA)

Sample Type: Cell Samples, Plasma, Tissue Samples, Urine

Analytical Method: Quantitative

Product Details

Detection Range: 0.28 U/L - 168.91 U/L

Minimum Detection Limit: 0.28 U/L

Purpose: Pyruvate Dehydrogenase (PDH) Activity Assay Kit

Characteristics: Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm.

Application Details

Assay Time: 15 min

Assay Precision:

Inter-assay precision: 10%

Intra-assay precision: 2%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: PDP (Pyruvate Dehydrogenase Phosphatase (PDP))

Alternative Name: PDH