Sirtuin 1 (SIRT-1) Activity Assay Kit
Quick Overview for Sirtuin 1 (SIRT-1) Activity Assay Kit (ABIN7850092)
Target
See all SIRT1 KitsDetection Method
Application
Sample Type
Analytical Method
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Detection Range
- 0.03 U/L - 9.66 U/L
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Minimum Detection Limit
- 0.03 U/L
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Purpose
- Sirtuin 1 (SIRT-1) Activity Assay Kit
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Characteristics
- This kit uses the fluorescence resonance energy transfer (FRET) method for detection. After deacetylation by SIRT-1, the fluorescent substrate generates an activated fluorescent substrate, which can be decomposed by proteases to produce fluorescent substances. The maximum excitation wavelength of fluorescent substances is 340 nm, and the maximum emission wavelength is 440 nm.
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Sirtuin 1 (SIRT1) ELISA Kit
SIRT1 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reactivity: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
SensoLyte® Green Sirt1 Assay KitFRET Fluorometric
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reactivity: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
SensoLyte® 520 FRET Sirt1 Assay KitFRET Fluorometric
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Assay Time
- 1 h
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Assay Precision
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Inter-assay precision: 8.1-9.9%
Intra-assay precision: 3.0-5.0%
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Restrictions
- For Research Use only
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Handling Advice
- Protect from light.
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Storage
- -20 °C
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Storage Comment
- This product can be stored at -20°C for 12 months with shading light.
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Expiry Date
- 12 months
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- SIRT1 (Sirtuin 1 (SIRT1))
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Alternative Name
- Sirtuin 1
Target
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