Sphingomyelin (SM) Fluorometric Assay Kit

Catalog No. ABIN7850093

Quick Overview for Sphingomyelin (SM) Fluorometric Assay Kit (ABIN7850093)

Target: Sphingomyelin (Sphingomyelin (SM))

Detection Method: Fluorometric

Application: Biochemical Assay (BCA)

Sample Type: Cell Samples, Plasma, Serum, Tissue Samples

Analytical Method: Quantitative

Product Details

Detection Range: 0.35 μmol/L - 40 μmol/L

Minimum Detection Limit: 0.35 μmol/L

Purpose: Sphingomyelin (SM) Fluorometric Assay Kit

Characteristics: Sphingomyelin (SM) is widely present in cell membranes and plasma lipoproteins. Metabolism of SM is regulated by sphingomyelin synthase and sphingomyelase, whose metabolites include ceramide, sphingosinol and choline phosphate, which are important second messengers. Studies have shown that SM and its metabolites play an important role in liver, lung, and cardiovascular diseases. The principle of this kit is that SM is catalyzed by sphingomyelin enzyme, and its products generate fluorescent substances with the probe under the action of other enzymes. By substituting the fluorescence value into the standard curve, SM content in the sample can be calculated.

Application Details

Assay Time: 1.5 h

Assay Precision:

Inter-assay precision: 6.0-10.0%

Intra-assay precision: 4.9-5.1%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: Sphingomyelin (Sphingomyelin (SM))

Alternative Name: Sphingomyelin