Coxsackie Adenovirus Receptor ELISA Kit

Catalog No. ABIN8055454

The Rat Coxsackie Adenovirus Receptor ELISA Kit (ABIN8055454) is a Colorimetric ELISA Kit designed to quantify Rat Coxsackie Adenovirus Receptor.

Quick Overview for Coxsackie Adenovirus Receptor ELISA Kit (ABIN8055454)

Target: Coxsackie Adenovirus Receptor (CXADR) (Coxsackie Virus and Adenovirus Receptor (CXADR))

Binding Specificity: AA 20-237

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Product Details Coxsackie Adenovirus Receptor ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich Rat CXADR ELISA Kit to quantitate Rat Cxadr in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: L20-G237

Capture antibody and Detection antibody: A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 6.1%, 4.7%, 5.6%
Inter-Assay Precision (CV%): 6.4%, 7.3%, 7.8%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

Target Details for Coxsackie Adenovirus Receptor

Target: Coxsackie Adenovirus Receptor (CXADR) (Coxsackie Virus and Adenovirus Receptor (CXADR))

Alternative Name: Cxadr

Background:

Background: CXADR (Coxsackie virus and adenovirus receptor) is a protein that in humans is encoded by the CXADR gene, also known as CAR,CVB3-binding protein, this gene is mapped to 11q11. Coxsackievirus B-adenovirus receptor. The CAR cDNA encodes a predicted 365-amino acid polypeptide that contains a single transmembrane domain and is a member of the immunoglobulin superfamily. By Northern blot analysis, they detected highest expression of 1.4-kb and 6-kb CXADR transcripts in pancreas, brain, heart, small intestine, testis, and prostate, lower expression in liver and lung, and no expression in kidney, placenta, peripheral blood leukocytes, thymus, and spleen. In comparison, mouse Cxadr showed highest expression in liver, and lower levels in kidney, heart, lung, and brain. The protein encoded by this gene is a type I membrane receptor for group B coxsackie viruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. CAR is strongly expressed in the developing central nervous system. It functions as a homophilic and also as a heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins , such as: fibronectin, agrin, laminin-1 and tenascin-R.

Gene Full Name: CXADR cell adhesion molecule

Gene ID: 89843

UniProt: Q9R066

Pathways: Cell-Cell Junction Organization