PSG1 ELISA Kit

Catalog No. ABIN8055571

The Human PSG1 ELISA Kit (ABIN8055571) is a Colorimetric ELISA Kit designed to quantify Human PSG1.

Quick Overview for PSG1 ELISA Kit (ABIN8055571)

Target: PSG1 (Pregnancy Specific beta-1-Glycoprotein 1 (PSG1))

Binding Specificity: AA 35-419

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine

Product Details PSG1 ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Purpose: Sandwich Human PSG1 ELISA Kit to quantitate Human PSG1 in cell culture supernatants, serum, plasma (heparin or EDTA) and urine.

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: Q35-P419

Capture antibody and Detection antibody: monoclonal antibody from mouse|monoclonal antibody from mouse

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 50 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.9%, 4.7%, 4.1%
Inter-Assay Precision (CV%): 5.6%, 5.1%, 6.6%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

Target Details for PSG1

Target: PSG1 (Pregnancy Specific beta-1-Glycoprotein 1 (PSG1))

Alternative Name: PSG1

Background:

Background: The PSG1 is a semi-automatic sniper rifle, Pregnancy-specific beta-1 glycoprotein 1 (PSG-1) is also known as SP1, PSbG1, or B1G1, and is designated CD66f. It is mapped to 19q13.2. The human placenta is a multihormonal endocrine organ that produces hormones, enzymes, and other molecules that support fetal survival and development. Pregnancy-specific beta-1-glycoprotein (PSBG, PSG) is a major product of the syncytiotrophoblast, reaching concentrations of 100 to 290 mg/l at term in the serum of pregnant women. PSG is a member of the immunoglobulin (Ig) superfamily.

Gene Full Name: pregnancy specific beta-1-glycoprotein 1

Gene ID: 5669

UniProt: P11464