Osteomodulin ELISA Kit

Catalog No. ABIN8055578

Human Osteomodulin ELISA Kit, Colorimetric assay for quantification of Human Osteomodulin.

Quick Overview for Osteomodulin ELISA Kit (ABIN8055578)

Target: Osteomodulin (OMD)

Binding Specificity: AA 21-421

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Product Details Osteomodulin ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich Human Osteoadherin ELISA Kit to quantitate Human OMD in cell culture supernatants, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: Q21-E421

Capture antibody and Detection antibody: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 5.5%, 6.6%, 7.3%
Inter-Assay Precision (CV%): 5.5%, 6.8%, 8.8%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

Target Details for Osteomodulin

Target: Osteomodulin (OMD)

Alternative Name: OMD

Background:

Background: Osteomodulin (also called osteoadherin or osteoadherin proteoglycan) is a protein that in humans is encoded by the OMD gene. This gene is mapped to 9q22.31. Osteoadherin (OSAD), also known as Osteomodulin, is an extracellular matrix keratan sulfate proteoglycan that belongs to the class II subfamily of small leucine-rich proteoglycans (SLRP). LRR motifs consist of approximately 20 30 amino acids (aa) with conserved leucine spacing, folded into a structure with one beta -sheet and one alpha -helix. Human OSAD is expressed as an even larger 110 kDa molecule in teeth. OSAD contains eight sulfated tyrosine residues and is distinguished from other class II SLRPs by the presence of an approximately 70 aa C terminal acidic domain. OSAD is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon. In dental tissue, OSAD is expressed by odontoblasts and ameloblasts and is involved in the mineralization of bone and teeth. OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3.

Gene Full Name: osteomodulin

Gene ID: 4958

UniProt: Q99983

Pathways: Glycosaminoglycan Metabolic Process