MEGF9 ELISA Kit

Catalog No. ABIN8055882

This Colorimetric ELISA kit is designed for the quantitative measurement of Human MEGF9.

Quick Overview for MEGF9 ELISA Kit (ABIN8055882)

Target: MEGF9 (Multiple EGF-Like-Domains 9 (MEGF9))

Binding Specificity: AA 36-514

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Product Details MEGF9 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich Human MEGF9 ELISA Kit to quantitate Human MEGF9 in cell culture supernatants, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: A36-N514

Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 75 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 5.8%, 6.2%, 3.1%
Inter-Assay Precision (CV%): 2.1%, 7.8%, 6.0%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

Target Details for MEGF9

Target: MEGF9 (Multiple EGF-Like-Domains 9 (MEGF9))

Alternative Name: MEGF9

Background:

Background: MEGF9 (Multiple EGF like domains protein 9, also EGF like protein 5) is a 63 kDa (predicted) novel transmembrane glycoprotein that shares some homology to beta chains of laminin. It is expressed by hepatocytes, cerebellar Purkinje cells, Schwann cells, keratinocytes and intestinal epithelium. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration. Mature human MEGF9 is 572 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 484 aa extracellular region (aa 31 514) plus a 68 aa C terminal cytoplasmic domain. The extracellular region possesses a lengthy Pro rich region (aa 55 200), followed by five EGF like domains (aa 204 451). MEGF9 may run at approximately 160 kDa in SDS PAGE, suggesting either heavy glycosylation or dimerization. Over aa 36 514, human MEGF9 shares 76 % aa sequence identity with mouse MEGF9.

Gene Full Name: multiple EGF like domains 9

Gene ID: 1955

UniProt: Q9H1U4