CEMIP ELISA Kit

Catalog No. ABIN8055930

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CEMIP.

Quick Overview for CEMIP ELISA Kit (ABIN8055930)

Target: CEMIP (Cell Migration Inducing Hyaluronan Binding Protein (CEMIP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.4 ng/mL - 90 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Product Details CEMIP ELISA Kit

Minimum Detection Limit: 0.4 ng/mL

Purpose: Sandwich Human Hyaluronan ELISA Kit to quantitate Human Hyaluronan in cell culture supernatants, serum, plasma(heparin, EDTA).

Analytical Method: Quantitative

Specificity:

Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 50 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 6.0%, 2.0%, 4.2%
Inter-Assay Precision (CV%): 5.4%, 4.0%, 2.0%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

Target Details for CEMIP

Target: CEMIP (Cell Migration Inducing Hyaluronan Binding Protein (CEMIP))

Alternative Name: CEMIP

Background:

Background: Cell migration-inducing and hyaluronan-binding protein (CEMIP), formerly known as KIAA1199, is a protein that in humans is encoded by the CEMIP gene. Enables several functions, including clathrin heavy chain binding activity, hyaluronic acid binding activity, and hyalurononglucosaminidase activity. Involved in several processes, including hyaluronan catabolic process, positive regulation of protein phosphorylation, and positive regulation of protein targeting to membrane. Located in several cellular components, including clathrin-coated endocytic vesicle, endoplasmic reticulum, and nucleus.

Gene ID: 57214

UniProt: Q8WUJ3