Hepcidin CLIA Kit

Catalog No. ABIN8056029

This Chemiluminescent ELISA kit is designed for the quantitative measurement of Human Hepcidin.

Quick Overview for Hepcidin CLIA Kit (ABIN8056029)

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Competition ELISA

Detection Range: 78.13 pg/mL - 20000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details Hepcidin CLIA Kit

Minimum Detection Limit: 78.13 pg/mL

Purpose: CLIA Kit for Hepcidin (Hepc)

Analytical Method: Quantitative

Specificity:

This assay has high sensitivity and excellent specificity for detection of Hepcidin (Hepc).
No significant cross-reactivity or interference between Hepcidin (Hepc) and analogues was observed.

Sensitivity: < 27.74 pg/mL

Components:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• Substrate A
• Substrate B
• Wash Buffer (30X)
• Instruction manual

Material not included:

• Luminometer capable of reading 96-well microplates with the following parameters: lag time 30.0secs; read time 1.0 sec/well
• Single or multi-channel pipettes with high precision and disposable tips.
• Microcentrifuge Tubes.
• Deionized or distilled water.
• Absorbent paper for blotting the microplate.
• Container for Wash Solution.
• 0.01mol/L (or 1×) Phosphate Buffered Saline(PBS), pH7.0-7.2

Application Details

Sample Volume: 50 μL

Assay Time: 2 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

Assay Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepcidin (Hepc) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepcidin (Hepc) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage: 4 °C,-20 °C

Storage Comment:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Expiry Date: 6 months

Target Details

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Alternative Name: Hepcidin

Background: HAMP, HFE2B, PLTR, LEAP1, Hepcidin Antimicrobial Peptide, Liver-expressed antimicrobial peptide 1, Putative liver tumor regressor

UniProt: P81172

Pathways: Hormone Activity, Transition Metal Ion Homeostasis