BTLA ELISA Kit

Catalog No. ABIN8056709

Human BTLA ELISA Kit, Colorimetric assay for quantification of Human BTLA.

Quick Overview for BTLA ELISA Kit (ABIN8056709)

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Grade: Small Sample

Product Details BTLA ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: Mini Samples ELISA Kit for B And T-Lymphocyte Attenuator (BTLA)

Analytical Method: Quantitative

Specificity:

This assay has high sensitivity and excellent specificity for detection of Mini Samples B And T-Lymphocyte Attenuator (BTLA).
No significant cross-reactivity or interference between Mini Samples B And T-Lymphocyte Attenuator (BTLA) and analogues was observed.

Sensitivity: < 0.059 ng/mL

Components:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Material not included:

• Microplate reader with 450 ± 10nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Application Details

Sample Volume: 25 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

Assay Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples B And T-Lymphocyte Attenuator (BTLA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples B And T-Lymphocyte Attenuator (BTLA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage: 4 °C,-20 °C

Storage Comment:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Expiry Date: 6 months

Target Details for BTLA

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Alternative Name: B And T-Lymphocyte Attenuator

Background: CD272, BTLA1, B-and T-lymphocyte-associated protein

UniProt: Q7Z6A9

Pathways: Cancer Immune Checkpoints