APOA2 ELISA Kit

Catalog No. ABIN8057089

The Human APOA2 ELISA Kit (ABIN8057089) is a Colorimetric ELISA Kit designed to quantify Human APOA2.

Quick Overview for APOA2 ELISA Kit (ABIN8057089)

Target: APOA2 (Apolipoprotein A-II (APOA2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.53 pg/mL - 500 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details APOA2 ELISA Kit

Minimum Detection Limit: 0.53 pg/mL

Analytical Method: Quantitative

Specificity: Apo A2

Components:

• Plate
• Standard
• Diluent

Material not included: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Apo A2.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: protect your eyes

Storage: 4 °C

Storage Comment: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Target Details for APOA2

Target: APOA2 (Apolipoprotein A-II (APOA2))

Alternative Name: Apo A2

Background: Apolipoprotein AII (Apo A2), the second most abundant protein after Apo A1 of human plasma high-density lipoproteins (HDL), is the most lipophilic of the exchangeable apolipoproteins. Apo A2 is synthesized by the liver and the small intestine and found in plasma as a monomer, dimer. The Apo A2 gene consists of 4 exons and 3 introns. The four exons encode the 5' untranslated region, pre peptide, a short N terminal domain and a C terminal domain composed of a variable number of lipid binding amphipathic helices. Apo A2 may stabilize HDL structure by its association with lipids, and affect the HDL metabolism. Role of Apo A2 in lipid metabolism and atherosclerosis show important clinical significance. Apo A2 levels have been associated with increased susceptibility to atherosclerosis, increased free fatty acid levels, increased body fat, and increased insulin resistance.

Gene ID: 336

NCBI Accession: NM_001643

UniProt: P02652

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Negative Regulation of Transporter Activity, Lipid Metabolism