Interleukin 17a ELISA Kit

Catalog No. ABIN8057095

The Mouse Interleukin 17a ELISA Kit (ABIN8057095) is a Colorimetric ELISA Kit designed to quantify Mouse Interleukin 17a.

Quick Overview for Interleukin 17a ELISA Kit (ABIN8057095)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.18 pg/mL - 100 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Grade: High Sensitivity

Product Details Interleukin 17a ELISA Kit

Minimum Detection Limit: 0.18 pg/mL

Analytical Method: Quantitative

Specificity: IL-17A

Components:

• Plate
• Standard
• Diluent

Material not included: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-17A.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: protect your eyes

Storage: 4 °C

Storage Comment: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Target Details for Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL-17A

Background: Interleukin 17 (IL-17) , also known as IL-17A and CTLA-8, is a homodimeric cytokine of about 32 kDa. IL-17 is a cytokine that acts as a potent mediator in delayed-type reactions by increasing chemokine production in various tissues to recruit monocytes and neutrophils to the site of inflammation, similar to IFN-γ. IL-17 is produced by T-helper cells and is induced by IL-23 which results in destructive tissue damage in delayed-type reactions. IL-17 as a family functions as a proinflammatory cytokine that responds to the invasion of the immune system by extracellular pathogens and induces destruction of the pathogen's cellular matrix. IL-17 acts synergistically with TNF and IL-1. IL-17 family has been linked to many immune/autoimmune related diseases including rheumatoid arthritis, asthma, lupus, allograft rejection, anti-tumour immunity and recently Psoriasis.Increased levels of IL-17 have been associated with several conditions, including airway inflammation, rheumatoid arthritis, intraperitoneal abscesses and adhesions, inflammatory bowel disease, allograft rejection, psoriasis, cancer and multiple sclerosis.

Gene ID: 16171

NCBI Accession: NM_010552

UniProt: Q62386