CA242 ELISA Kit

Catalog No. ABIN8065412

Human CA242 ELISA Kit, Colorimetric assay for quantification of Human CA242.

Quick Overview for CA242 ELISA Kit (ABIN8065412)

Target: CA242 (Carbohydrate Antigen 242 (CA242))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL – 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Product Details CA242 ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Purpose: In vitro quantitative determination of ca242 concentrations in serum, plasma, cell culture supernatant

Analytical Method: Quantitative

Specificity: Specifically recognize ca242, no obvious cross reaction with other analogues

Sensitivity: 18.75 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Material not included: - Microplate reader (wavelength: 450nm)
- 37 °C incubator
- Automated plate washer
- Precision single and multi-channel pipette and disposable tips
- Clean tubes and Eppendorf tubes
- Deionized or distilled water

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Assay Time: 4 h

Plate: Pre-coated

Protocol: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ca242 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti ca242 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ca242 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ca242 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

Assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate. CV% = <6%
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.CV%= <7%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Target Details for CA242

Target: CA242 (Carbohydrate Antigen 242 (CA242))

Alternative Name: CA242

Background: CA242(Pancreatic Carcinoma Markers-CA242)