NAA10 ELISA Kit

Catalog No. ABIN8068013

Human NAA10 ELISA Kit, Colorimetric assay for quantification of Human NAA10.

Quick Overview for NAA10 ELISA Kit (ABIN8068013)

Target: NAA10 (ARD1A) (ARD1 Homolog, N-Acetyltransferase (ARD1A))

Binding Specificity: acetylated, total

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Lysate, Tissue Lysate

Product Details NAA10 ELISA Kit

Purpose: Measures acetylated NAA10 and total NAA10 in human cell and tissue lysates, enabling precise assessment of NAA10 acetylation by ELISA. This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.

Analytical Method: Quantitative

Components:

Size / Qty
• Microplate Coated With Anti-Pan-NAA10 Antibody
• Anti-Acetylation Detection Antibody
• Anti-NAA10 Detection Antibody
• HRP-Conjugated Streptavidin
• 200 μL
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Log-log graph paper or computer and software for ELISA data analysis
• Absorbent paper
• Tubes to prepare sample dilutions
• Distilled or deionized water
• Shaker

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed.
2. Add 100 μL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Target Details for NAA10

Target: NAA10 (ARD1A) (ARD1 Homolog, N-Acetyltransferase (ARD1A))

Alternative Name: NAA10

Background:

Gene Symbols: NAA10, ARD1, ARD1A, TE2

Background: The Acetylated-NAA10 and Total-NAA10 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human acetylated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan NAA10 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and NAA10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-acetylation antibody is used to detect acetylated NAA10 or biotinylated anti-NAA10 antibody is used to detect total NAA10 . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of acetylated NAA10 or total NAA10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): N-alpha-acetyltransferase 10, N-terminal acetyltransferase complex ARD1 subunit homolog A (hARD1), NatA catalytic subunit Naa10

Gene ID: 8260

UniProt: P41227