Corticosterone ELISA Kit

Catalog No. ABIN8068523

Human, Rabbit, Pig, Dog, Sheep, Cat and Horse Corticosterone ELISA Kit, Colorimetric assay for quantification of Human, Rabbit, Pig, Dog, Sheep, Cat and Horse Corticosterone.

Quick Overview for Corticosterone ELISA Kit (ABIN8068523)

Target: Corticosterone (CORT)

Reactivity: Human, Rabbit, Pig, Dog, Sheep, Cat, Horse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.0001 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Saliva, Serum, Urine

Product Details Corticosterone ELISA Kit

Minimum Detection Limit: 0.0001 ng/mL

Purpose: This kit is a competitive immunoassay that quantifies corticosterone in serum or plasma from human, porcine, feline, canine, rabbit, equine, and sheep samples (not rat or mouse) within 2.5 hours.

Analytical Method: Quantitative

Specificity: This EIA kit detects Corticosterone

Sensitivity: 0.0021 ng/mL

Components:

• Pre-Coated 96-well Strip Microplate
• Assay Diluent
• Lyophilized Standard
• Positive Control
• Capture Antibody
• HRP-conjugated secondary antibody
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• Software which can perform four-parameter logistic regression models

Application Details

Application Notes: Suggested Plasma Dilution: 2-4 folds

Sample Volume: 50 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
3. Add 50 μL Capture Antibody to appropriate well. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 5-20 minutes at room temperature or 37 °C.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Target Details for Corticosterone

Target: Corticosterone (CORT)

Alternative Name: Corticosterone

Target Type: Hormone

Background: Optional[Gene Symbols]: Corticosterone, a glucocorticoid synthesized in the adrenal cortex upon ACTH stimulation, serves as both the biosynthetic precursor of aldosterone and a key biomarker of stress. Stress-induced elevations in corticosterone have been linked to impaired long-term memory retrieval, chronic increases following dietary restriction, and marked rises after burn injury. Beyond its role in the stress response, corticosterone also helps shape sleep-wake cycles.

The Corticosterone Enzyme Immunoassay (EIA) Kit (EIA-CORT2) is a competitive immunoassay that quantifies corticosterone in serum or plasma from human, porcine, feline, canine, rabbit, equine, and sheep samples (not rat or mouse) within 2.5 hours. Absorbance is measured at 450 nm and values are interpolated from a ready-to-use corticosterone standard curve. The assay demonstrates a sensitivity of 0.0021 ng/mL and has been validated for linearity, stability, precision, and spike-recovery performance. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.