CRH ELISA Kit

Catalog No. ABIN8068526

Mouse, Human and Rat CRH ELISA Kit, Colorimetric assay for quantification of Mouse, Human and Rat CRH.

Quick Overview for CRH ELISA Kit (ABIN8068526)

Target: CRH (Corticotropin Releasing Hormone (CRH))

Reactivity: Mouse, Human, Rat

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.016 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details CRH ELISA Kit

Minimum Detection Limit: 0.016 ng/mL

Purpose: Competitive ELISA (EIA) kit for detecting Corticotropin Releasing Factor (CRF), also known as Corticotropin Releasing Hormone (CRH), in serum and cell culture medium samples.

Analytical Method: Quantitative

Specificity: This EIA kit is designed to detect human, mouse, and rat Corticotropin Releasing Factor

Sensitivity: 0.1 ng/mL

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Application Details

Application Notes: Suggested Plasma Dilution: Human: 4X / Mouse: 2X / Rat: 2X

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

• Prepare all reagents, samples and standards as instructed.
• Add 100 μL detection antibody to each well.
• Incubate 1.5 h at RT or O/N at 4 °C.
• Add 100 μL standard or sample to each well.
• Incubate 2.5 h at RT.
• Add 100 μL prepared streptavidin solution.
• Incubate 45 min at RT.
• Add 100 μL TMB One-Step Substrate Reagent to each well.
• Incubate 30 min at RT.
• Add 50 μL Stop Solution to each well.
• Read plate at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Standard, Biotinylated CRF / CRH peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Target Details for CRH

Target: CRH (Corticotropin Releasing Hormone (CRH))

Alternative Name: Corticotropin Releasing Factor

Background:

Gene Symbols: CRH

Background: Corticotropin-releasing factor (CRF) (also known as Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Its main function is the stimulation of the pituitary synthesis of adrenocorticotropic hormone (ACTH), as part of the hypothalamic-pituitary-adrenal axis (HPA axis). CRF levels may be associated with Alzheimer"s disease and major depression. CRF is also synthesized in peripheral tissues, such as T lymphocytes, and is highly expressed in the placenta, suggesting roles in parturition.

The RayBiotech CRF Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting CRF peptide based on the competitive enzyme immunoassay principle. In this assay, a biotinylated CRF peptide is spiked into the samples and standards. The samples and standards are then added to the plate, where the biotinylated CRF peptide competes with endogenous (unlabeled) CRF for binding to the anti-CRF antibody. After a wash step, any bound biotinylated CRF then interacts with horseradish peroxidase (HRP)-streptavidin, which catalyzes a color development reaction. The intensity of the colorimetric signal is directly proportional to the amount of captured biotinylated CRF peptide and inversely proportional to the amount of endogenous CRF in the standard or samples. A standard curve of known concentration of CRF peptide can be established and the concentration of CRF peptide in the samples can be calculated accordingly.

Protein Name (Synonyms): Corticotropin-releasing hormone (CRH), corticotropin-releasing factor (CRF), corticoliberin

Gene ID: 1392

UniProt: P06850

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour