CRYAB ELISA Kit

Catalog No. ABIN8068538

Human CRYAB ELISA Kit, Colorimetric assay for quantification of Human CRYAB.

Quick Overview for CRYAB ELISA Kit (ABIN8068538)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Detection Range: 1.37 units/mL - 1000 units/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details CRYAB ELISA Kit

Minimum Detection Limit: 1.37 units/mL

Purpose: The Alpha-B crystallin (CRYAB) Human IgG ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgG antibody against pathogen antigen(s) in human serum or plasma.

Analytical Method: Quantitative

Components:

Size / Description
• Cryab Coated 96-Well Microplate
• 96 wells coated with CRYAB
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgG
• HRP-Streptavidin
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent
• Sample Diluent

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm.
• Precision pipettes to deliver 2 μL to 1 mL volumes.
• Adjustable 1-25 mL pipettes for reagent preparation.
• 100 mL and 1 liter graduated cylinders.
• Absorbent paper.
• Distilled or deionized water.

Application Details

Application Notes: Suggested Plasma Dilution: 1000-fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL positive control, or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into respective wells. Incubate 1 hour at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: CRYAB

Background:

Gene Symbols: CRYAB CRYA2 HSPB5

Background: The Alpha-B crystallin (CRYAB) Human IgG ELISA Kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with a pathogen antigen, which combines with the corresponding antibody present in a sample and Positive Control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgG antibody is added, a complex forms between biotinylated secondary antibody and human antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-pathogen antigen antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

The Positive Control consists of an anti-Alpha-B crystallin antibody. A biotinylated anti-Positive Control antibody is added to the Positive Control wells and treated identically to the samples. The intensity of the resulting color (measured at 450 nm) is used as a calibration curve for interpretation purposes in the different assays.

Protein Name (Synonyms): Alpha-crystallin B chain (Alpha(B)-crystallin) (Heat shock protein beta-5) (HspB5) (Renal carcinoma antigen NY-REN-27) (Rosenthal fiber component)

Gene ID: 1410

UniProt: P02511