Digoxin ELISA Kit

Catalog No. ABIN8068666

The Human, Cow and Mouse Digoxin ELISA Kit (ABIN8068666) is a Colorimetric ELISA Kit designed to quantify Human, Cow and Mouse Digoxin.

Quick Overview for Digoxin ELISA Kit (ABIN8068666)

Target: Digoxin (DIG)

Reactivity: Human, Cow, Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.01 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Saliva, Serum, Urine

Product Details Digoxin ELISA Kit

Minimum Detection Limit: 0.01 ng/mL

Purpose: This kit is a competitive immunoassay that quantifies DIG in serum or plasma from human, mouse, rat, equine, and bovine samples, as well as urine samples within 2.5 hours.

Analytical Method: Quantitative

Specificity: This EIA kit detects Digoxin

Sensitivity: 0.0427 ng/mL

Components:

Size / Description
• DIG Microplate
• 96 wells coated with DIG
• Assay Diluent
• Standard DIG
• Positive Control
• Anti-DIG Antibody
• ~10 μL
• HRP-conjugated Secondary Antibody
• ~5 μL
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• GraphPad Prism or SigmaPlot software

Application Details

Application Notes: Suggested Plasma Dilution: 2-4 folds

Sample Volume: 50 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
3. Add 50 μL anti-DIG to appropriate well. Incubate 1 hour at room temperature. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10-25 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Target Details for Digoxin

Target: Digoxin (DIG)

Alternative Name: Digoxin

Target Type: Chemical

Background: Optional[Gene Symbols]: Digoxin (DIG) is a once-daily medication used to treat certain heart conditions when first-line therapies are ineffective or when additional treatment is required. Because digoxin has a narrow therapeutic index, careful monitoring is essential. Excessive digoxin levels can lead to dangerous, and sometimes fatal, cardiac arrhythmias. Therefore, serum digoxin testing helps ensure that drug levels are sufficient to provide therapeutic benefit while avoiding toxicity.

The DIG Enzyme Immunoassay (EIA) Kit is a competitive immunoassay that quantifies DIG in serum or plasma from human, mouse, rat, equine, and bovine samples, as well as urine samples within 2.5 hours. Absorbance is measured at 450 nm, and concentrations are interpolated from a ready-to-use DIG standard curve. The assay demonstrates a sensitivity of 0.0427 ng/mL and has been validated for linearity, stability, precision, and spike-recovery performance. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.