Estradiol ELISA Kit

Catalog No. ABIN8068770

This Colorimetric ELISA kit is designed for the quantitative measurement of Human, Sheep, Dog, Horse, Pig, Rabbit and Cat Estradiol.

Quick Overview for Estradiol ELISA Kit (ABIN8068770)

Target: Estradiol

Reactivity: Human, Sheep, Dog, Horse, Pig, Rabbit, Cat

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.01 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Urine

Product Details Estradiol ELISA Kit

Minimum Detection Limit: 0.01 pg/mL

Purpose: Estradiol Enzyme Immunoassay Kit is a competitive enzyme immunoassay developed for rapid E2 detection within 2.5 hours in serum and plasma samples collected from various species.

Analytical Method: Quantitative

Sensitivity: 0.09 pg/mL

Components:

• Pre-Coated 96-well Strip Microplate
• Assay Diluent
• Lyophilized Standard
• Positive Control
• Capture Antibody
• HRP-conjugated secondary antibody
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• Software which can perform four-parameter logistic regression models

Application Details

Application Notes: Suggested Plasma Dilution: 2-3 folds

Sample Volume: 50 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
3. Add 50 μL Capture Antibody to appropriate well. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 5-20 minutes at room temperature or 37 °C.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Target Details for Estradiol

Target: Estradiol

Target Type: Hormone

Background: Optional[Gene Symbols]: Estradiol (E2) is an estrogen steroid hormone, also known as 17β-estradiol (to distinguish it from 17α-estradiol) or as estra-1,3,5(10)-triene-3,17β-diol. It is the major female sex hormone, and involved in the regulation of the estrous and menstrual female reproductive cycles. Estradiol is responsible for the female sexual development, and is important in the development and maintenance of female reproductive tissues. Estradiol is produced mainly by the granulosa cells of the ovaries, is also in other tissues including the testicles, the adrenal glands, fat, liver, the breasts, and the brain.