Fibrinogen ELISA Kit

Catalog No. ABIN8068828

The Human Fibrinogen ELISA Kit (ABIN8068828) is a Colorimetric ELISA Kit designed to quantify Human Fibrinogen.

Quick Overview for Fibrinogen ELISA Kit (ABIN8068828)

Target: Fibrinogen

Binding Specificity: citrullinated

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.5625 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Lysate

Product Details Fibrinogen ELISA Kit

Minimum Detection Limit: 1.5625 ng/mL

Purpose: Citrullinated Fibrinogen Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Citrullinated Fibrinogen (Fgn).

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human Citrullinated Fibrinogen.

Sensitivity: 0.19 ng/mL

Components:

Size / Qty
• Anti-Pan-Fgn Microplate
• 96 wells
• Citrullinated-Fgn Standard
• Anti-Citrullinated Fgn Detection Antibody
• HRP-conjugated anti-mouse IgG concentrate
• 25 μL
• Wash Buffer
• Assay Diluent B
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Log-log graph paper or computer and software for ELISA data analysis
• Absorbent paper
• Tubes to prepare positive control or sample dilutions
• Distilled or deionized water
• Shaker

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL of each standard and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-conjugated anti-mouse IgG solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Target Details for Fibrinogen

Target: Fibrinogen

Background:

Gene Symbols: FGB

Background: The Citrullinated Fibrinogen Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Citrullinated Fibrinogen (Fgn). An anti-pan fibrinogen antibody has been coated onto a 96-well plate. Standard and Samples are pipetted into the wells and fibrinogen present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-citrullinated fibrinogen antibody is used to detect Citrullinated Fibrinogen. After washing away unbound antibody, HRP-conjugated anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Citrullinated Fibrinogen bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): Fibrinogen beta chain [Cleaved into: Fibrinopeptide B, Fibrinogen beta chain]

Gene ID: 2244

UniProt: P02675