GLP-1 ELISA Kit

Catalog No. ABIN8068907

Human, Dog, Rabbit, Pig, Sheep, Cat and Horse GLP-1 ELISA Kit, Colorimetric assay for quantification of Human, Dog, Rabbit, Pig, Sheep, Cat and Horse GLP-1.

Quick Overview for GLP-1 ELISA Kit (ABIN8068907)

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Reactivity: Human, Dog, Rabbit, Pig, Sheep, Cat, Horse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.01 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Urine

Product Details GLP-1 ELISA Kit

Minimum Detection Limit: 0.01 pg/mL

Purpose: This kit specifically measures the active GLP-1 forms (7-36 and 7-37 amide), and does not detect the original (1-37 amide) or inactive (9-36 and 9-37 amide) forms.

Analytical Method: Quantitative

Sensitivity: 0.071 pg/mL

Components:

• Pre-Coated 96-well Strip Microplate
• Assay Diluent
• Lyophilized Standard
• Positive Control
• Capture Antibody
• HRP-conjugated secondary antibody
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• Software which can perform four-parameter logistic regression models

Application Details

Application Notes: Suggested Plasma Dilution: 2-4 folds

Sample Volume: 50 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature or 37 °C.
3. Add 50 μL Capture Antibody to appropriate well. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 2-10 minutes at room temperature or 37 °C.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Target Details for GLP-1

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Alternative Name: GLP-1

Background:

Gene Symbols: GCG

Background: Glucagon-like peptide-1 (GLP-1) is a highly conserved amino-acid-long peptide hormone among mammals and mainly secreted from the intestinal L-cells and the CNS. Functionally, GLP-1 plays an important role in energy homeostasis and nutrient absorption. The initial product GLP-1 (1-37) is susceptible to amidation and proteolytic cleavage, which gives rise to the two truncated or equipotent biologically active forms, GLP-1 (7-36) and GLP-1 (7-37) amide. Then, the active forms of GLP-1 are extremely susceptible to the catalytic activity of the proteolytic enzyme dipeptidyl peptidase-4 (DPP-4), resulting in the abundant inactivated GLP-1 (9-36) or GLP-1 (9-37) amide constituting 60-80 % of total GLP-1 in circulation. This kit could specifically measure the active (7-36 and 7-37 amide) GLP-1, while not the original (1-37 amide) and inactive (9-36 and 9-37 amide) forms of GLP-1.

The active GLP-1 Enzyme Immunoassay (EIA) Kit (EIA-AGLP1) is a competitive enzyme immunoassay developed for rapid detection and quantitation of active GLP-1 in serum and plasma samples collected from various species including humans, porcine, feline, canine, rabbit, equine and sheep, but not rats or mice. The quantity of active GLP-1 is determined by a known standard GLP-1 curve. The active GLP-1 kit has a high sensitivity of approximately 0.071 ng/mL. The performance was also verified to exhibit good characteristics in terms of linearity, stability, precision, and spiking & recovery. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.

Protein Name (Synonyms): Pro-glucagon [Cleaved into: Glicentin, Glicentin-related polypeptide (GRPP), Oxyntomodulin (OXM) (OXY), Glucagon, Glucagon-like peptide 1 (GLP-1) (Incretin hormone), Glucagon-like peptide 1(7-37) (GLP-1(7-37)), Glucagon-like peptide 1(7-36) (GLP-1(7-36)), Glucagon-like peptide 2 (GLP-2)]

Gene ID: 2641

UniProt: P01275