GLP-1 ELISA Kit

Catalog No. ABIN8068910

Human, Dog, Rabbit, Pig, Sheep, Cat and Horse GLP-1 ELISA Kit, Colorimetric assay for quantification of Human, Dog, Rabbit, Pig, Sheep, Cat and Horse GLP-1.

Quick Overview for GLP-1 ELISA Kit (ABIN8068910)

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Reactivity: Human, Dog, Rabbit, Pig, Sheep, Cat, Horse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.01 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Urine

Product Details GLP-1 ELISA Kit

Minimum Detection Limit: 0.01 pg/mL

Purpose: This kit specifically measures the inactive GLP-1 forms (9-36 and 9-37 amide), and does not detect the original (1-37 amide) or active (7-36 and 7-37 amide) forms.

Analytical Method: Quantitative

Specificity: This EIA kit detects GLP-1 (ZGLP1)

Sensitivity: 0.663 pg/mL

Components:

• Pre-Coated 96-well Strip Microplate
• Assay Diluent
• Lyophilized Standard
• Positive Control
• Capture Antibody
• HRP-conjugated secondary antibody
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• Software which can perform four-parameter logistic regression models

Application Details

Application Notes: Suggested Plasma Dilution: 2-4 folds

Sample Volume: 50 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
3. Add 50 μL Capture Antibody to appropriate well. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature or 37 °C. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 5-20 minutes at room temperature or 37 °C.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Target Details for GLP-1

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Alternative Name: GLP-1

Background:

Gene Symbols: GCG

Background: Glucagon-like peptide-1 (GLP-1) is a highly conserved amino-acid-long peptide hormone among mammals and mainly secreted from the intestinal L-cells and the CNS. Functionally, GLP-1 plays an important role in energy homeostasis and nutrient absorption. The initial product GLP-1 (1-37) is susceptible to amidation and proteolytic cleavage, which gives rise to the two truncated or equipotent biologically active forms, GLP-1 (7-36) and GLP-1 (7-37) amide. Then, the inactive forms of GLP-1 are extremely susceptible to the catalytic activity of the proteolytic enzyme dipeptidyl peptidase-4 (DPP-4), resulting in the abundant inactivated GLP-1 (9-36) or GLP-1 (9-37) amide constituting 60-80 % of total GLP-1 in circulation. This kit could specifically measure the inactive (9-36 and 9-37 amide) GLP-1, while not the original (1-37 amide) and active (7-36 and 7-37 amide) forms of GLP-1.

The inactive GLP-1 Enzyme Immunoassay (EIA) Kit (EIA-AGLP1) is a competitive enzyme immunoassay developed for rapid detection and quantitation of inactive GLP-1 in serum and plasma samples collected from various species including humans, porcine, feline, canine, rabbit, equine and sheep, but not rats or mice. The quantity of inactive GLP-1 is determined by a known standard GLP-1 curve. The inactive GLP-1 kit has a high sensitivity of approximately 0.663 pg/mL. The performance was also verified to exhibit good characteristics in terms of linearity, stability, precision, and spiking & recovery. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.

Protein Name (Synonyms): Pro-glucagon [Cleaved into: Glicentin, Glicentin-related polypeptide (GRPP), Oxyntomodulin (OXM) (OXY), Glucagon, Glucagon-like peptide 1 (GLP-1) (Incretin hormone), Glucagon-like peptide 1(7-37) (GLP-1(7-37)), Glucagon-like peptide 1(7-36) (GLP-1(7-36)), Glucagon-like peptide 2 (GLP-2)]

Gene ID: 2641

UniProt: P01275