GLP-1 ELISA Kit

Catalog No. ABIN8068911

This Colorimetric ELISA kit is designed for the quantitative measurement of Human, Mouse and Rat GLP-1.

Quick Overview for GLP-1 ELISA Kit (ABIN8068911)

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Binding Specificity: total

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.1 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details GLP-1 ELISA Kit

Minimum Detection Limit: 0.1 pg/mL

Purpose: Human/Mouse/Rat total Glucagon-like peptide-1 EIA Kit for optimized for serum and cell culture medium. Competition-based ELISA

Analytical Method: Quantitative

Specificity: This kit measures total GLP-1, including the full-length (1-37), active (7-36), and inactive (9-36) forms. The antibody shows similarly high affinity for each form. No cross-reactivity observed with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.

Sensitivity: 0.288 pg/mL

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Lyophilized Standard
• Capture Antibody
• Assay Diluent
• Biotinylated Peptide
• HRP-Streptavidin
• Positive Control
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Software which can perform four-parameter logistic regression models
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• Aluminum foil
• Plastic wrap

Application Details

Application Notes: Suggested Plasma Dilution: Human: 2X / Mouse: 2X / Rat: 2X

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL Capture Antibody to each well. Incubate 1.5 hours at room temperature or overnight at 4 °C.
3. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C.
4. Add 100 μL prepared Streptavidin solution. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Target Details for GLP-1

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Alternative Name: GLP-1

Background:

Gene Symbols: GCG

Background: Glucagon-like peptide-1 (GLP-1) is a highly conserved amino-acid-long peptide hormone among mammals and mainly secreted from the intestinal L-cells and the CNS. Functionally, GLP-1 plays an important role in energy homeostasis and nutrient absorption. The initial product GLP-1 (1-37) is susceptible to amidation and proteolytic cleavage, which gives rise to the two truncated and equipotent biologically active forms, GLP-1 (7-36) amide and GLP-1 (7-37). Then, the active forms of GLP-1 are extremely susceptible to the catalytic activity of the proteolytic enzyme dipeptidyl peptidase-4 (DPP-4), resulting in the abundant inactivated GLP-1 (9-36) amide constituting 60-80 % of total GLP-1 in circulation. This kit measures total GLP-1 including full length GLP-1 (1-37), the active (7-36) and inactive (9-36) forms of GLP-1.

Protein Name (Synonyms): Pro-glucagon [Cleaved into: Glicentin, Glicentin-related polypeptide (GRPP), Oxyntomodulin (OXM) (OXY), Glucagon, Glucagon-like peptide 1 (GLP-1) (Incretin hormone), Glucagon-like peptide 1(7-37) (GLP-1(7-37)), Glucagon-like peptide 1(7-36) (GLP-1(7-36)), Glucagon-like peptide 2 (GLP-2)]

Gene ID: 2641

UniProt: P01275