HDAC1 ELISA Kit

Catalog No. ABIN8069030

Human and Mouse HDAC1 ELISA Kit, Colorimetric assay for quantification of Human and Mouse HDAC1.

Quick Overview for HDAC1 ELISA Kit (ABIN8069030)

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Binding Specificity: SUMOylated

Reactivity: Human, Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Lysate, Tissue Lysate

Product Details HDAC1 ELISA Kit

Purpose: Sumoylated-HDAC1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse Sumoylated-HDAC1 and HDAC1 in lysate samples.

Analytical Method: Quantitative

Components:

Size / Qty
• Anti-Pan-HDAC1 Microplate
• 96 wells
• Positive Control
• Anti-Sumoylation Detection Antibody
• Anti-HDAC1 Detection Antibody
• HRP-Streptavidin
• 200 μL
• Wash Buffer 1
• Assay Diluent B
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Log-log graph paper or computer and software for ELISA data analysis
• Absorbent paper
• Tubes to prepare positive control or sample dilutions
• Distilled or deionized water
• Shaker

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and Positive Control as instructed.
2. Add 100 μLsample and Positive Control to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Target Details for HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Alternative Name: HDAC1

Background:

Gene Symbols: HDAC1, RPD3L1

Background: The Sumoylated-HDAC1 and Total-HDAC1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse sumoylated histone deacetylase 1 and total histone deacetylase 1. An anti-pan HDAC1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and HDAC1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-sumoylation antibody is used to detect sumoylated HDAC1 or biotinylated anti-HDAC1 antibody is used to detect total HDAC1. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of sumoylated HDAC1 or total HDAC1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): Histone deacetylase 1 (HD1) (EC 3.5.1.98) (Protein deacetylase HDAC1) (EC 3.5.1.-) (Protein decrotonylase HDAC1) (EC 3.5.1.-)

Gene ID: 3065433759

UniProt: Q13547, O09106

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis