IgG ELISA Kit
Quick Overview for IgG ELISA Kit (ABIN8069312)
Target
See all IgG ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- COVID-19 Spike Variant Human IgG ELISA Kit 13 is a human antibody detection kit (indirect ELISA method) for the quantitative measurement of human IgG against the following spike protein mutations of SARS-CoV-2: N440D. This Product is for Research Use Only. Not for use in in vitro diagnostics.
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Analytical Method
- Semi-Quantitative
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Components
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- SARS-CoV-2 Protein coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Anti-Human IgG
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
- SARS-CoV-2 Protein coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Anti-Human IgG
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
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Material not included
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Distilled or deionized water
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IgG ELISA Kit
Reactivity: Rabbit Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Rabbit TrueBlot® Set (with IP beads)WB, IP Reactivity: Rabbit
Mouse TrueBlot® Set (with IP beads)WB, IP Reactivity: Mouse
IgG ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Cow Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivity: Mouse Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Human Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivity: Chicken Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivity: Pig Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
- Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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Restrictions
- For Research Use only
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Storage
- -80 °C
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Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.
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- IgG
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Target Type
- Antibody
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Background
- Gene Symbols: IGG
Target See all IgG ELISA Kits
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