IgM ELISA Kit
Quick Overview for IgM ELISA Kit (ABIN8069642)
Target
See all IgM ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- COVID-19 N and S1 RBD protein Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgM antibody against SARS-CoV-2 in serum. This Product is for Research Use Only. Not for use in in vitro diagnostics.
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Analytical Method
- Semi-Quantitative
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Components
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- SARS-CoV-2 N-protein coated 96-well microplate
- Wash Buffer
- Positive Control
- Negative Control
- HRP-anti positive control IgM Antibody
- HRP-anti Human IgM Antibody
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- SARS-CoV-2 N-protein coated 96-well microplate
- Wash Buffer
- Positive Control
- Negative Control
- HRP-anti positive control IgM Antibody
- HRP-anti Human IgM Antibody
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
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Material not included
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
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IgM ELISA Kit
Reactivity: Chicken Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
IgM ELISA KitReactivity: Human Colorimetric Sandwich ELISA 31.2 ng/mL - 2000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Grouper (Epinephelinae) Colorimetric Competition ELISA 1.25-50 μg/mL Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Cow Colorimetric Competition ELISA 0.39 μg/mL - 25 μg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Goat Colorimetric Sandwich ELISA 0.234 ng/mL - 15 ng/mL Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Rabbit Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Sheep Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Cow Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgM ELISA KitReactivity: Horse Colorimetric Sandwich ELISA 10 ng/mL - 320 ng/mL Plasma, Serum, Urine
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL positive control, negative control, or sample to each well. Incubate 1.5 hours at room temperature.
- Add 100 μL prepared HRP-anti positive control IgM Antibody and/or 100 μL of prepared HRP-anti Human IgM Antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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Restrictions
- For Research Use only
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Storage
- -80 °C
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Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.
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- IgM
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Target Type
- Antibody
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Background
- Gene Symbols: IGM
Target See all IgM ELISA Kits
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