MANF ELISA Kit

Catalog No. ABIN8070037

The Human MANF ELISA Kit (ABIN8070037) is a Colorimetric ELISA Kit designed to quantify Human MANF.

Quick Overview for MANF ELISA Kit (ABIN8070037)

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.013 ng/mL - 5 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details MANF ELISA Kit

Minimum Detection Limit: 0.013 ng/mL

Purpose: Human MANF ELISA kit for Serum and Plasma samples.

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human MANF. Other species not determined.

Components:

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard and sample dilutions

Application Details

Application Notes: Suggested Plasma Dilution: 2-10 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Target Details for MANF

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: MANF

Background:

Gene Symbols: MANF

Protein Name (Synonyms): Mesencephalic astrocyte-derived neurotrophic factor (Arginine-rich protein) (Protein ARMET)

Gene ID: 7873

UniProt: P55145