Nuclease ELISA Kit

Catalog No. ABIN8070291

This Colorimetric ELISA kit is designed for the quantitative measurement of Serratia marcescens Nuclease.

Quick Overview for Nuclease ELISA Kit (ABIN8070291)

Target: Nuclease

Reactivity: Serratia marcescens

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 19.92 pg/mL - 9600 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant

Product Details Nuclease ELISA Kit

Minimum Detection Limit: 19.92 pg/mL

Purpose: Nuclease ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of nucleases, including SuperNuclease® and Benzonase®, in cell culture supernatants and purified biological products while nuclease is supplied to remove host cell DNA during manufacturing.

Analytical Method: Quantitative

Specificity: This ELISA kit has been validated with SuperNuclease®, Benzonase® and RayBio® Universal Nuclease, and should react with other Serratia marcescens nuclease products but not tested. Both non-denatured and denatured nuclease can be detected by this kit.

Components:

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard and sample dilutions

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Target Details for Nuclease

Target: Nuclease

Background:

Gene Symbols: nucA nuc

Protein Name (Synonyms): Nuclease (EC 3.1.30.2) (Endonuclease) [Cleaved into: Nuclease isoform Sm2, Nuclease isoform Sm3, Nuclease isoform Sm1

UniProt: P13717