Nucleoprotein ELISA Kit

Catalog No. ABIN8070304

The Human Nucleoprotein ELISA Kit (ABIN8070304) is a Colorimetric ELISA Kit designed to quantify Human Nucleoprotein.

Quick Overview for Nucleoprotein ELISA Kit (ABIN8070304)

Target: Nucleoprotein

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Plasma, Serum

Product Details

Purpose: SFTS Virus Nucleoprotein (NP) Human IgM ELISA kit is an in-vitro indirect ELISA for the quantitative measurement of Human IgM autoantibody against NP in serum or plasma samples. This product is for research use only. Not for therapeutic or diagnostic applications.

Analytical Method: Quantitative

Components:

• Pre-coated 96-well Microplate
• Wash Buffer
• Positive Control
• Biotinylated Secondary Antibody
• HRP-Streptavidin Concentrate
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent
• Sample Diluent

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL Positive Control or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Secondary Antibody into respective wells. Incubate 1 hour at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Target Details

Target: Nucleoprotein

Target Type: Influenza Protein

Background:

Background: This < span class="italic-txt">in-vitro ELISA kit employs an indirect ELISA method. In this kit, standard 96-well plates are coated with the pathogen antigen, which combines with the corresponding antibody present in a sample and positive control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgA/G/M antibody is added, a complex forms between the biotinylated secondary antibody and human antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-pathogen antigen antibody present. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The intensity of the resulting color (measured at 450 nm) is used as a calibration curve for interpretation purposes in the different assays.

Protein Name (Synonyms): Nucleoprotein, Nucleocapsid protein

Gene ID: 13231122

UniProt: F1BA48