Peroxiredoxin 6 ELISA Kit

Catalog No. ABIN8070378

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Peroxiredoxin 6.

Quick Overview for Peroxiredoxin 6 ELISA Kit (ABIN8070378)

Target: Peroxiredoxin 6 (PRDX6)

Binding Specificity: SUMOylated, total

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Lysate, Tissue Lysate

Product Details Peroxiredoxin 6 ELISA Kit

Purpose: Measures sumoylated Peroxiredoxin 6 (PRDX6) and total Peroxiredoxin 6 in human cell and tissue lysates, enabling precise assessment of Peroxiredoxin 6 sumoylation by ELISA. This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.

Analytical Method: Quantitative

Components:

Size / Qty
• Microplate Coated With Anti-Pan-Peroxiredoxin 6 Antibody
• Anti-SUMOylation Detection Antibody
• Anti-Peroxiredoxin 6 Detection Antibody
• HRP-Conjugated Streptavidin
• 200 μL
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Log-log graph paper or computer and software for ELISA data analysis
• Absorbent paper
• Tubes to prepare sample dilutions
• Distilled or deionized water
• Shaker

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed.
2. Add 100 μL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Target Details for Peroxiredoxin 6

Target: Peroxiredoxin 6 (PRDX6)

Alternative Name: Peroxiredoxin-6

Background:

Gene Symbols: PRDX6, AOP2, KIAA0106

Background: The SUMOylated-Peroxiredoxin 6 and Total-Peroxiredoxin 6 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human sumoylated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan Peroxiredoxin 6 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Peroxiredoxin 6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-sumoylation antibody is used to detect sumoylated Peroxiredoxin 6 or biotinylated anti-Peroxiredoxin 6 antibody is used to detect total Peroxiredoxin 6 . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of sumoylated Peroxiredoxin 6 or total Peroxiredoxin 6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): Peroxiredoxin-6, 1-Cys peroxiredoxin (1-Cys PRX), 24 kDa protein, Acidic calcium-independent phospholipase A2 (aiPLA2), Antioxidant protein 2, Glutathione-dependent peroxiredoxin, Liver 2D page spot 40, Lysophosphatidylcholine acyltransferase 5 (LPC acyltransferase 5, LPCAT-5, Lyso-PC acyltransferase 5), Non-selenium glutathione peroxidase (NSGPx), Red blood cells page spot 12

Gene ID: 9588

UniProt: P30041