PGE2 ELISA Kit

Catalog No. ABIN8070523

The Mouse, Human and Rat PGE2 ELISA Kit (ABIN8070523) is a Colorimetric ELISA Kit designed to quantify Mouse, Human and Rat PGE2.

Quick Overview for PGE2 ELISA Kit (ABIN8070523)

Target: PGE2 (Prostaglandin E2 (PGE2))

Reactivity: Mouse, Human, Rat

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.01 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details PGE2 ELISA Kit

Minimum Detection Limit: 0.01 pg/mL

Purpose: Human/Mouse/Rat Prostaglandin E2 (PGE2) EIA Kit optimized for serum and Plasma. Competition-based ELISA

Analytical Method: Quantitative

Specificity: This EIA kit is designed to detect human, mouse, and rat PGE2.

Sensitivity: 0.1386 pg/mL

Components:

• Pre-Coated 96-well Strip Microplate
• Assay Diluent(s)
• Standard Peptide
• Positive Control Sample
• Capture Antibody
• HRP-conjugated secondary antibody
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• GraphPad Prism or SigmaPlot software

Application Details

Application Notes: Suggested Plasma Dilution: Human: 4X / Mouse: 2X / Rat: 2X

Sample Volume: 50 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
3. Add 50 μL anti-PGE2 to appropriate well. Incubate 1 hour at room temperature. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 2-10 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Target Details for PGE2

Target: PGE2 (Prostaglandin E2 (PGE2))

Alternative Name: Prostaglandin E2

Background:

Gene Symbols: PGE2

Background: Prostaglandin E2 (PGE2), also known as dinoprostone, has important effects in labor by inducing softening of the cervix and causing uterine contraction, and also stimulating osteoblasts to release factors that stimulate bone resorption by osteoclasts. It is important in the structure and function of the ductus arteriosus in fetuses and newborns. PGE2 also has a variety of functions within the Central Nervous System and Peripheral Nervous System. It contributes to the inflammation when bound to EP2 receptors. PGE2 serves a significant role in vascular smooth muscle tone regulation. Within the kidney, the function of PGE2 is to maintain renal blood flow and glomerular filtration rate (GFR) through localized vasodilation.

The PGE2 ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of PGE2 in serum and plasma samples collected from various species including humans, rats or mice. The quantity of PGE2 is determined by a known standard PGE2 curve. The PGE2 kit has a high sensitivity of approximately 0.1386 ng/mL. The performance was also verified to exhibit good characteristics in terms of linearity, stability, precision, and spiking & recovery. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.

Protein Name (Synonyms): Prostaglandin E2, PGE2, dinoprostone, (5Z,11α,13E,15S)-11,15-Dihydroxy-9-oxo-prosta-5,13-dien-1-oic acid, Prostin E2, Cervidil, Propess