tau ELISA Kit

Catalog No. ABIN8070981

This Colorimetric ELISA kit is designed for the quantitative measurement of Human, Mouse and Rat tau.

Quick Overview for tau ELISA Kit (ABIN8070981)

Target: tau (tau Protein)

Binding Specificity: pThr217

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.37 ng/mL - 90 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Lysate

Product Details tau ELISA Kit

Minimum Detection Limit: 0.37 ng/mL

Purpose: Phospho-TAU (Thr217) Quantitative ELISA Kit is a convenient and sensitive assay to detect phosphorylated Tau at Threonine-217 (p-Tau217) in human cell lysate samples.

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human, mouse and rat Phospho TAU (Thr217).

Sensitivity: 0.09 ng/mL

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Target Details for tau

Target: tau (tau Protein)

Alternative Name: Tau

Background:

Gene Symbols: MAPT MAPTL MTBT1 TAU

Protein Name (Synonyms): Microtubule-associated protein tau (Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)

Gene ID: 4137

UniProt: P10636