tau ELISA Kit

Catalog No. ABIN8070985

The Human, Mouse and Rat tau ELISA Kit (ABIN8070985) is a Colorimetric ELISA Kit designed to quantify Human, Mouse and Rat tau.

Quick Overview for tau ELISA Kit (ABIN8070985)

Target: tau (tau Protein)

Binding Specificity: pThr231

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.064 ng/mL - 90 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Lysate

Product Details tau ELISA Kit

Minimum Detection Limit: 0.064 ng/mL

Purpose: Phospho-TAU (Thr231) Quantitative ELISA Kit is a convenient and sensitive assay to detect phosphorylated Tau at Threonine-231 in human, mouse, rat cell lysate samples.

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human, mouse and rat Phospho TAU (Thr231).

Components:

• Pre-coated 96-well microplate
• Lyophilized Standard
• Anti-phospho Detection Antibody
• HRP-Conjugated Secondary Antibody
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Log-log graph paper or computer and software for ELISA data analysis
• Absorbent paper
• Tubes to prepare the positive control or sample dilutions
• Distilled or deionized water
• Shaker

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and positive control as instructed.
2. Add 100 μL of each standard and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-conjugated secondary antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Target Details for tau

Target: tau (tau Protein)

Alternative Name: Tau

Background:

Gene Symbols: MAPT, MAPTL, MTBT1, TAU

Background: Phospho-TAU (Thr231) Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human, Mouse and Rat phosphorylated TAU (Thr231). An anti-pan TAU antibody has been coated onto a 96-well plate. Standard and Samples are pipetted into the wells and TAU present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-TAU (Thr231) antibody is used to detect phosphorylated TAU. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TAU (Thr231) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau (PHF-tau)

Gene ID: 413717762

UniProt: P10636, P19332, P10637