Triiodothyronine T3 ELISA Kit

Catalog No. ABIN8071153

The Human, Rat, Dog, Mouse, Pig, Sheep, Horse and Cat Triiodothyronine T3 ELISA Kit (ABIN8071153) is a Colorimetric ELISA Kit designed to quantify Human, Rat, Dog, Mouse, Pig, Sheep, Horse and Cat Triiodothyronine T3.

Quick Overview for Triiodothyronine T3 ELISA Kit (ABIN8071153)

Target: Triiodothyronine T3 (T3)

Reactivity: Human, Rat, Dog, Mouse, Pig, Sheep, Horse, Cat

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.002 ng/dL - 2000 ng/dL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Saliva, Serum, Urine

Product Details Triiodothyronine T3 ELISA Kit

Minimum Detection Limit: 0.002 ng/dL

Purpose: This kit is a competitive immunoassay designed to quantify reverse T3 in human, mouse, rat, porcine, feline, canine, equine, and sheep serum or plasma, as well as urine samples, within 2.5 hours.

Analytical Method: Quantitative

Specificity: This EIA kit detects Triiodothyronine / T3

Sensitivity: 0.042 ng/dL

Components:

• Pre-Coated 96-well Strip Microplate
• Assay Diluent
• Lyophilized Standard
• Positive Control
• Capture Antibody
• HRP-conjugated Secondary Antibody
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• Software which can perform four-parameter logistic regression models

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 50 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
3. Add 50 μL Capture Antibody to appropriate well. Incubate 1 hour at room temperature. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary Antibody solution. Incubate 1 hour at room temperature. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 5-20 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Target Details for Triiodothyronine T3

Target: Triiodothyronine T3 (T3)

Alternative Name: Triiodothyronine

Target Type: Amino Acid

Background: Optional[Gene Symbols]: Reverse T3 (rT3) is an inactive metabolite of thyroxine (T4). It is generated when T4 is converted by type 3 deiodinase (D3) rather than being transformed into the biologically active form of T3. Unlike T3, rT3 does not activate thyroid hormone receptors and therefore has no metabolic activity.

Measurement of rT3 is useful for determining whether thyroid hormone is being shunted away from active metabolism during periods of physiological stress or illness. Clinically, elevated rT3 levels are commonly observed in severe or chronic illness (such as infection, trauma, or surgery), starvation or very low-calorie diets, chronic inflammation, liver or kidney disease, and with certain medications (e.g., corticosteroids and amiodarone). In contrast, low rT3 levels are generally not clinically significant when interpreted on their own.

The Reverse T3 Enzyme Immunoassay (EIA) Kit is a competitive immunoassay that quantifies T3 (rT3) in serum or plasma from human, mouse, rat, porcine, feline, canine, equine, and sheep samples, as well as in urine samples, within 2.5 hours. Absorbance is measured at 450 nm and values are interpolated from a ready-to-use rT3 standard curve. The assay demonstrates a sensitivity of 0.042 ng/dL and has been validated for linearity, stability, precision, and spike-recovery performance. Each kit provides sufficient reagents to perform up to 96 assays, including the standard curve and unknown samples.