TRIM21 ELISA Kit

Catalog No. ABIN8071161

This Colorimetric ELISA kit is designed for the quantitative measurement of Human TRIM21.

Quick Overview for TRIM21 ELISA Kit (ABIN8071161)

Target: TRIM21 (Tripartite Motif Containing 21 (TRIM21))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Detection Range: 1.37 units/mL - 1000 units/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details TRIM21 ELISA Kit

Minimum Detection Limit: 1.37 units/mL

Purpose: The Sjogren Syndrome Antigen (SSA) Human IgA ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgA antibody against SSA antigen(s) in human serum or plasma.

Analytical Method: Quantitative

Components:

Size / Description
• SSA Coated 96-Well Microplate
• 96 wells coated with SSA
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgA
• HRP-Streptavidin
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent
• Sample Diluent

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water

Application Details

Application Notes: Suggested Plasma Dilution: 1,000-fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control, or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Anti-Human IgA Antibody into respective wells. Incubate 1 hour at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The unopened kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles.

Target Details for TRIM21

Target: TRIM21 (Tripartite Motif Containing 21 (TRIM21))

Alternative Name: TRIM21

Background:

Gene Symbols: TRIM21 RNF81 RO52 SSA1

Background: The Sjogren Syndrome Antigen (SSA) Human IgA ELISA Kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with SSA protein, which combines with the corresponding antibody present in a sample and Positive Control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgA antibody is added, a complex forms between biotinylated secondary antibody and human antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-SSA antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): E3 ubiquitin-protein ligase TRIM21 (EC 2.3.2.27) (52 kDa Ro protein) (52 kDa ribonucleoprotein autoantigen Ro/SS-A) (RING finger protein 81) (Ro(SS-A)) (Sjoegren syndrome type A antigen) (SS-A) (Tripartite motif-containing protein 21)

Gene ID: 6737

UniProt: Q5XPV5, Q96RF8