HVD3 ELISA Kit
Quick Overview for HVD3 ELISA Kit (ABIN8073800)
Target
See all HVD3 (25-HVD3) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 2.47 ng/mL
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Purpose
- 25-Hydroxyvitamin D3 (HVD3) ELISA Kit for detecting HVD3 in serum, plasma
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Analytical Method
- Quantitative
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Sensitivity
- 0.91 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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25-Hydroxy Vitamin D3 (25-HVD3) ELISA Kit
25-HVD3 Reactivity: Various Species Colorimetric Competition ELISA 6.17 ng/mL - 500 ng/mL Plasma, Serum
25-Hydroxy Vitamin D3 (25-HVD3) ELISA Kit25-HVD3 Reactivity: Rat Colorimetric Competition ELISA 20-100 μg/L Serum
25-Hydroxy Vitamin D3 (25-HVD3) ELISA Kit25-HVD3 Reactivity: Human Colorimetric Competition ELISA 20-100 μg/L Serum
25-Hydroxy Vitamin D3 (25-HVD3) ELISA Kit25-HVD3 Reactivity: Mouse Colorimetric Competition ELISA 20-100 μg/L Serum
25-Hydroxy Vitamin D3 (25-HVD3) ELISA Kit25-HVD3 Reactivity: Chemical Colorimetric Competition ELISA 3.13 ng/mL - 200 ng/mL Plasma, Serum
25-Hydroxy Vitamin D3 (25-HVD3) ELISA Kit25-HVD3 Reactivity: Monkey Colorimetric Competition ELISA 40-200 μg/L Plasma, Serum, Tissue Homogenate
25-Hydroxy Vitamin D3 (25-HVD3) ELISA Kit25-HVD3 Reactivity: Guinea Pig Colorimetric Competition ELISA 20-100 μg/L Plasma, Serum, Tissue Homogenate
25-Hydroxy Vitamin D3 (25-HVD3) ELISA Kit25-HVD3 Reactivity: Various Species Colorimetric Competition ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 50 μL
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Assay Time
- 2 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- HVD3 (25-HVD3) (25-Hydroxy Vitamin D3 (25-HVD3))
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Alternative Name
- 25-Hydroxyvitamin D3
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Target Type
- Chemical
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Background
- Gene Aliases: 25-HVD3, 25HVD3, Calcifediol, Calcidiol, H-VD3, 25-Hydroxycholecalciferol, 25-Hydroxy Vitamin D3, 25 (OH) D
Target See all HVD3 (25-HVD3) ELISA Kits
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