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Alkaline Phosphatase CLIA Kit

Cow Alkaline Phosphatase ELISA Kit, Chemiluminescent assay for quantification of Cow Alkaline Phosphatase.
Catalog No. ABIN8074321
$1,138.50
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for Alkaline Phosphatase CLIA Kit (ABIN8074321)

Target

See all Alkaline Phosphatase (ALP) CLIA Kits
Alkaline Phosphatase (ALP)

Reactivity

  • 14
  • 10
  • 9
  • 6
  • 6
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cow

Detection Method

Chemiluminescent

Method Type

Sandwich ELISA

Detection Range

68.6 pg/mL - 50000 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
  • Minimum Detection Limit

    68.6 pg/mL

    Purpose

    Bovine Alkaline Phosphatase (ALP) CLIA Kit for detecting ALP in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

    Analytical Method

    Quantitative

    Sensitivity

    25.3 pg/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • Substrate A
    • Substrate B
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all Alkaline Phosphatase (ALP) CLIA Kits

    Alkaline Phosphatase (ALP)

    Alternative Name

    Alkaline Phosphatase

    Background

    Gene Aliases: AKP, ALKP, Basic Phosphatase

    Gene ID

    280994

    UniProt

    P09487
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