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APP ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human, Rat and Mouse APP.
Catalog No. ABIN8074469
$812.88
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for APP ELISA Kit (ABIN8074469)

Target

See all APP ELISA Kits
APP (Amyloid beta (A4) Precursor Protein (APP))

Binding Specificity

pThr668, pThr743

Reactivity

  • 23
  • 16
  • 15
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
Human, Rat, Mouse

Detection Method

Colorimetric

Method Type

Cell ELISA

Application

ELISA

Sample Type

Cell Culture Cells
  • Purpose

    Phospho-Amyloid β A4 (Thr743/668) Colorimetric Cell-Based ELISA Kit for detecting Phospho-Amyloid β A4 (Thr743/668) in researchers plate their cell line of choice.

    Analytical Method

    Qualitative

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Assay Time

    4 - 5 h

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    6 months
  • Target See all APP ELISA Kits

    APP (Amyloid beta (A4) Precursor Protein (APP))

    Alternative Name

    Amyloid beta A4

    Background

    Gene Aliases: Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II, APP, A4, AD1

    Gene ID

    351

    UniProt

    P05067

    Pathways

    Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour
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