Angiotensin I Converting Enzyme 1 ELISA Kit

Catalog No. ABIN8074590

The Mouse Angiotensin I Converting Enzyme 1 ELISA Kit (ABIN8074590) is a Colorimetric ELISA Kit designed to quantify Mouse Angiotensin I Converting Enzyme 1.

Quick Overview for Angiotensin I Converting Enzyme 1 ELISA Kit (ABIN8074590)

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate

Product Details Angiotensin I Converting Enzyme 1 ELISA Kit

Minimum Detection Limit: 0.312 ng/mL

Purpose: Mouse Angiotensin I Converting Enzyme (ACE) ELISA Kit for detecting ACE in serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates

Analytical Method: Quantitative

Sensitivity: 0.142 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for Angiotensin I Converting Enzyme 1

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Alternative Name: Angiotensin I Converting Enzyme

Background: Gene Aliases: CD143, ACE1, DCP1, ACEI, ACE-I, Kininase II, Angiotensin-Converting Enzyme, Peptidyl-Dipeptidase A, Dipeptidyl Carboxypeptidase 1, Angiotensin-converting enzyme, soluble form

Gene ID: 11421

UniProt: P09470

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration