ATG5 ELISA Kit

Catalog No. ABIN8075025

The Human ATG5 ELISA Kit (ABIN8075025) is a Colorimetric ELISA Kit designed to quantify Human ATG5.

Quick Overview for ATG5 ELISA Kit (ABIN8075025)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details ATG5 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Purpose: Human Autophagy Related Protein 5 (ATG5) Mini sample ELISA Kit for detecting ATG5 in serum, plasma, tissue homogenates

Analytical Method: Quantitative

Sensitivity: 0.28 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 15 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: Autophagy Related Protein 5

Background: Gene Aliases: ASP, APG5, APG5L, hAPG5, Apoptosis-specific protein, Autophagy protein 5

Gene ID: 9474

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy