BSP ELISA Kit
Quick Overview for BSP ELISA Kit (ABIN8075356)
Target
See all BSP ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 125 pg/mL
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Purpose
- Human Bone Sialoprotein (BSP) ELISA Kit for detecting BSP in serum, plasma, tissue homogenates
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Analytical Method
- Quantitative
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Sensitivity
- 50 pg/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Bone Sialoprotein (BSP) ELISA Kit
BSP Reactivity: Human Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Bone Sialoprotein (BSP) ELISA KitBSP Reactivity: Mouse Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Bone Sialoprotein (BSP) ELISA KitBSP Reactivity: Rat Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Bone Sialoprotein (BSP) ELISA KitBSP Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Bone Sialoprotein (BSP) ELISA KitBSP Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Bone Sialoprotein (BSP) ELISA KitBSP Reactivity: Rat Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- BSP (Bone Sialoprotein (BSP))
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Alternative Name
- Bone Sialoprotein
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Background
- Gene Aliases: IBSP, Bsp, BspII, SpII, Integrin-Binding Bone Sialoprotein, Cell-binding sialoprotein, Bone sialoprotein 2
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Gene ID
- 3381
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UniProt
- P21815
Target See all BSP ELISA Kits
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