CRAF ELISA Kit

Catalog No. ABIN8075504

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CRAF.

Quick Overview for CRAF ELISA Kit (ABIN8075504)

Target: CRAF (C-Raf Proto-Oncogene Serine/Threonine Protein Kinase (CRAF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details CRAF ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: Human C-Raf Proto Oncogene Serine/Threonine Protein Kinase (Raf-1) ELISA Kit for detecting Raf-1 in tissue homogenates, cell lysates

Analytical Method: Quantitative

Sensitivity: 0.059 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for CRAF

Target: CRAF (C-Raf Proto-Oncogene Serine/Threonine Protein Kinase (CRAF))

Alternative Name: C-Raf Proto Oncogene Serine/Threonine Protein Kinase

Background: Gene Aliases: RAF1, CRAF, V-raf-1 Murine Leukemia Viral Oncogene Homolog 1, Proto-oncogene c-RAF

Gene ID: 5894

UniProt: P04049