Calreticulin ELISA Kit

Catalog No. ABIN8075705

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Calreticulin.

Quick Overview for Calreticulin ELISA Kit (ABIN8075705)

Target: Calreticulin (CALR)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 2.47 ng/mL - 200 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details Calreticulin ELISA Kit

Minimum Detection Limit: 2.47 ng/mL

Purpose: Human Calreticulin (CALR) ELISA Kit for detecting CALR in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Sensitivity: 0.87 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 50 μL

Assay Time: 2 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: Calreticulin

Background: Gene Aliases: CRT, RO, SSA, CC1qR, ERp60, HACBP, grp60, CRTC, CRP55, Calregulin, Sicca Syndrome Antigen A, Autoantigen Ro, Endoplasmic reticulum resident protein 60

Gene ID: 811

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response