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Caspase 12 ELISA Kit

Mouse Caspase 12 ELISA Kit, Colorimetric assay for quantification of Mouse Caspase 12.
Catalog No. ABIN8075953
$859.38
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for Caspase 12 ELISA Kit (ABIN8075953)

Target

See all Caspase 12 (CASP12) ELISA Kits
Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Reactivity

  • 10
  • 9
  • 6
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.312 ng/mL - 20 ng/mL

Application

ELISA

Sample Type

Cell Lysate, Tissue Homogenate
  • Minimum Detection Limit

    0.312 ng/mL

    Purpose

    Mouse Caspase 12 (CASP12) ELISA Kit for detecting CASP12 in tissue homogenates, cell lysates

    Analytical Method

    Quantitative

    Sensitivity

    0.112 ng/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all Caspase 12 (CASP12) ELISA Kits

    Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

    Alternative Name

    Caspase 12

    Background

    Gene Aliases: CASP12P1, Cysteinyl Aspartate Specific Proteinases 12, Apoptosis-Related Cysteine Peptidase

    Gene ID

    12364

    UniProt

    O08736

    Pathways

    Apoptosis, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
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