CEBPB ELISA Kit

Catalog No. ABIN8076116

This Colorimetric ELISA kit is designed for the quantitative measurement of Human, Mouse and Rat CEBPB.

Quick Overview for CEBPB ELISA Kit (ABIN8076116)

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Binding Specificity: pThr188, pThr235

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details CEBPB ELISA Kit

Purpose: Phospho-C/EBP-β (Thr235/188) Colorimetric Cell-Based ELISA Kit for detecting Phospho-C/EBP-β (Thr235/188) in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for CEBPB

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Alternative Name: C/EBP-beta

Background: Gene Aliases: AGP/EBP, C/EBP beta, C/EBP-related protein 2, CCAAT/enhancer binding protein beta, CEBPB, CRP2, IL-6DBP, Interleukin-6- dependent binding protein, LAP, Liver-enriched transcriptional activator, Nuclear factor NF-IL6, SF-B, SFB, Silencer factor B, TCF5, Transcription factor 5

Gene ID: 1051

UniProt: P17676

Pathways: Interferon-gamma Pathway, Autophagy, Brown Fat Cell Differentiation