CD40 ELISA Kit
Quick Overview for CD40 ELISA Kit (ABIN8076200)
Target
See all CD40 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.156 ng/mL
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Purpose
- Human Tumor Necrosis Factor Receptor Superfamily, Member 5 (Cd40) Mini Sample ELISA Kit for detecting CD40 in tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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Sensitivity
- 0.054 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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CD40 (CD40) ELISA Kit
CD40 Reactivity: Human Colorimetric Sandwich ELISA 19.531 pg/mL - 1250 pg/mL Plasma, Serum, Tissue Homogenate
CD40 (CD40) ELISA KitCD40 Reactivity: Mouse Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
CD40 (CD40) ELISA KitCD40 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD40 (CD40) ELISA KitCD40 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD40 (CD40) ELISA KitCD40 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 (CD40) ELISA KitCD40 Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
CD40 (CD40) ELISA KitCD40 Reactivity: Mouse AA 24-193 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
CD40 (CD40) ELISA KitCD40 Reactivity: Human AA 21-193 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
CD40 (CD40) ELISA KitCD40 Reactivity: Rat Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum
CD40 (CD40) ELISA KitCD40 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 15 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- CD40
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Alternative Name
- Tumor Necrosis Factor Receptor Superfamily, Member 5
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Background
- Gene Aliases: TNFRSF5, CDW40, Bp50, P50, B-cell surface antigen CD40, CD40L receptor
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Gene ID
- 958
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UniProt
- P25942
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Pathways
- NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
Target See all CD40 ELISA Kits
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