Choline Acetyltransferase CLIA Kit
Quick Overview for Choline Acetyltransferase CLIA Kit (ABIN8076791)
Target
See all Choline Acetyltransferase (CHAT) CLIA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
-
-
Minimum Detection Limit
- 13.7 pg/mL
-
Purpose
- Rat Choline Acetyltransferase (ChAT) CLIA Kit for detecting ChAT in tissue homogenates
-
Analytical Method
- Quantitative
-
Sensitivity
- 5.9 pg/mL
-
Components
-
- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- Substrate A
- Substrate B
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
-
Material not included
-
- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
-
-
Choline O-Acetyltransferase (CHAT) ELISA Kit
CHAT Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitCHAT Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Lysate, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitCHAT Reactivity: Mouse Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitCHAT Reactivity: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitCHAT Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitCHAT Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitCHAT Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitCHAT Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitCHAT Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitSmall Sample CHAT Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Comment
-
This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
-
Sample Volume
- 100 μL
-
Assay Time
- 3 h
-
Plate
- Pre-coated
-
Assay Precision
-
Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
Storage Comment
- 4°C for one month, Long term: see manual.
-
Expiry Date
- 12 months
-
-
- Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
-
Alternative Name
- Choline Acetyltransferase
-
Background
- Gene Aliases: CMS1A, CMS1A2, CHOACTase, Choline acetylase
-
UniProt
- P32738
-
Pathways
- Skeletal Muscle Fiber Development
Target See all Choline Acetyltransferase (CHAT) CLIA Kits
-
