Prothrombin ELISA Kit
Quick Overview for Prothrombin ELISA Kit (ABIN8076962)
Target
See all Prothrombin (F2) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 125 ng/mL
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Purpose
- Bovine F2 ELISA Kit for detecting Coagulation Factor II in Serum, Plasma
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Analytical Method
- Quantitative
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Sensitivity
- 44 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Coagulation Factor II (thrombin) (F2) ELISA Kit
F2 Reactivity: Rat Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor II (thrombin) (F2) ELISA KitF2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor II (thrombin) (F2) ELISA KitF2 Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma
Coagulation Factor II (thrombin) (F2) ELISA KitF2 Reactivity: Human Colorimetric Sandwich ELISA 0.41 ng/mL - 300 ng/mL Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 Reactivity: Cow Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 Reactivity: Human Fragment 1 Colorimetric Sandwich ELISA 0.03-2 nM/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor II (thrombin) (F2) ELISA KitF2 Reactivity: Human Colorimetric Indirect ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Coagulation Factor II (thrombin) (F2) ELISA KitF2 Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
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Alternative Name
- Coagulation Factor II
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Background
- Gene Aliases: FII, TM, PT, Thrombin, Prothrombin, Pro-Thrombin, Activation peptide fragment 1, Activation peptide fragment 2
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Gene ID
- 280685
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UniProt
- P00735
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Pathways
- Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling
Target See all Prothrombin (F2) ELISA Kits
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