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Prothrombin ELISA Kit

The Human Prothrombin ELISA Kit (ABIN8076963) is a Colorimetric ELISA Kit designed to quantify Human Prothrombin.
Catalog No. ABIN8076963
$772.15
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for Prothrombin ELISA Kit (ABIN8076963)

Target

See all Prothrombin (F2) ELISA Kits
Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Reactivity

  • 17
  • 13
  • 12
  • 8
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.41 ng/mL - 300 ng/mL

Application

ELISA

Sample Type

Serum
  • Minimum Detection Limit

    0.41 ng/mL

    Purpose

    Human Coagulation Factor II (F2) ELISA Kit for detecting F2 in serum.

    Analytical Method

    Quantitative

    Sensitivity

    0.19 ng/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all Prothrombin (F2) ELISA Kits

    Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

    Alternative Name

    Coagulation Factor II

    Background

    Gene Aliases: FII, TM, PT, Thrombin, Prothrombin, Pro-Thrombin, Activation peptide fragment 1, Activation peptide fragment 2

    Gene ID

    2147

    UniProt

    P00734

    Pathways

    Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling
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