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Factor VIII ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat Factor VIII.
Catalog No. ABIN8077010
$1,119.58
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for Factor VIII ELISA Kit (ABIN8077010)

Target

See all Factor VIII (F8) ELISA Kits
Factor VIII (F8) (Coagulation Factor VIII (F8))

Reactivity

  • 17
  • 11
  • 7
  • 6
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

78 pg/mL - 5000 pg/mL

Application

ELISA

Sample Type

Plasma
  • Minimum Detection Limit

    78 pg/mL

    Purpose

    Rat Coagulation Factor VIII (F8) ELISA Kit for detecting F8 in plasma.

    Analytical Method

    Quantitative

    Sensitivity

    31 pg/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all Factor VIII (F8) ELISA Kits

    Factor VIII (F8) (Coagulation Factor VIII (F8))

    Alternative Name

    Coagulation Factor VIII

    Background

    Gene Aliases: FVIII, AHF, HEMA, F8B, F8C, Antihemophilic Globulin, Hemophilia A, Anti-Hemophilic Factor A, FVIII Procoagulant Component, F8 Protein

    UniProt

    D3ZEB1
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